TOP    Events & Outreach    R-CCS Cafe    The 235th R-CCS Cafe - part 3

Title

Three-dimensional structural determination of proteins from fluctuation X-ray scattering data

Details
Date Fri, Sep 16, 2022
Time 4:40 pm - 5:00 pm (5 pm - 5:20 pm Discussion, 5:20 pm - Free discussion (optional))
City Online
Place

Online seminar on BlueJeans

  • If you are not affiliated with R-CCS and would like to attend R-CCS Cafe, please email us at r-ccs-cafe[at]ml.riken.jp.
Language Presentation Language: English
Presentation Material: English
Speakers

Wenyang ZHAO

Computational Structural Biology Research Team
Postdoctoral Researcher

Abstract

Cellular functions, such as intracellular transport/mitosis/movement with microtubules, transmembrane ion/molecule transport with channel proteins, and transcription with ribosomes, are mostly realized by polymerization, depolymerization, and conformational change of proteins and biological complexes. In general, “seeing” their structures is the prerequisite to understanding their dynamics and functions. While well-established techniques such as X-ray crystallography and cryo-electron microscopy investigate their structures in crystallized or frozen conditions, fluctuation X-ray scattering (FXS) utilizes X-ray Free-Electron Laser (XFEL) and can measure non-crystallized biological particles dispersed in solutions in near-physiological conditions. This emerging technique is not only experimentally challenging but also has a theoretical difficulty of interpreting experimental data. In the experiment, thousands or millions of 2D X-ray coherent diffraction images are collected. Each image averages the information of many randomly oriented biological particles. Reconstruction to 3D structures requires not only an explicit mathematical theory but also high-performance computation. In this talk, I will introduce my recent progress of theoretical work. Results with simulated experimental data will be shown.

Important Notes

  • Please turn off your video and microphone when you join the meeting.
  • The broadcasting may be interrupted or terminated depending on the network condition or any other unexpected event.
  • The program schedule and contents may be modified without prior notice.
  • Depending on the utilized device and network environment, it may not be able to watch the session.
  • All rights concerning the broadcasted material will belong to the organizer and the presenters, and it is prohibited to copy, modify, or redistribute the total or a part of the broadcasted material without the previous permission of RIKEN.

(Sep 8, 2022)